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首页> 外文OA文献 >Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi
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Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi

机译：脂液滴中的中性脂质含量：冬虫夏宾蛋白生真菌中冬虫氏素积累的潜在生物标志物

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To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R2 = 0.9514) and in lyophilized mycelium powder (R2 = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R2 = 0.9727) and in lyophilized mycelium powder (R2 = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R2 = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation; these neutral lipids are potential biomarkers of cordycepin biosynthesis.
机译：为了阐明中性脂质含量与冬虫夏草中肠道堆之间的关系，突变体由两个C. MINILARIS菌株的混合孢子产生，具有不同的冬虫氏蛋白生产能力。最终选择加入0.001至2.363mg / ml冬虫蛋白的十五个稳定突变体。然后使用尼罗红方法研究了15个突变体，两个C. militaris菌株的相对荧光强度和不同浓度的冻干菌丝粉末的曲霉菌株。选择突变体Cm1-1-1-1-1-1-1-1-1-1-1-1-1，以优化尼罗红色方法。相对荧光强度与液体肉汤（R2 = 0.9514）中的冬虫夏素浓度线性相关，并且在相同的培养条件下，在肠肠蛋白产生的菌株和液体肉汤中的冬虫蛋白浓度（R2 = 0.9727）中，冬氏素粉末（R2 = 0.9378）。（R2 = 0.9727）在冻干的菌丝菌丝膜（R2 = 0.9613），在八个不同的条件下CM1-1-1。此外，通过尼罗红方法测量的冻干菌丝粉的肠素含量与通过HPLC方法确定的肠道粉末线性相关（R2 = 0.9627）。总之，在冬虫蛋白堆积期间需要在脂质液滴中的中性脂质;这些中性脂质是冬虫氏素生物合成的潜在生物标志物。

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