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A molecular assay for the proactive detection of target site-based resistance to herbicides inhibiting acetolactate synthase in Alopecurus myosuroides

机译：用于在alopecurus myosuroides中抑制丙酮酸合酶的靶位位点的靶位点的抗性的主动性检测的分子测定

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Acetolactate synthase (ALS) inhibitors are the most resistance-prone herbicide group. Rapid resistance diagnosis is thus of importance for their optimal use. We formulate rules to use the derived cleaved amplified polymorphic sequence method to develop molecular tools detecting a change at a given codon, the nature of which is unknown. We applied them to Alopecurus myosuroides (black grass) to develop assays targeting ALS codons A122, P197, A205, W574 and S653 that are crucial for herbicide sensitivity. These assays detected W574L or P197T, or both substitutions, in most plants analysed from a field where ALS inhibitors failed after 3 years of use. Similar assays can easily be set up for any species. Given the rapidity of selection for resistance to ALS inhibitors, these assays should be very useful in proactive herbicide resistance diagnosis.

机译：乙酰乳酸合酶（ALS）抑制剂是最抗性的易于除草剂基团。因此，快速的抗性诊断是对其最佳用途的重要性。我们制定规则以使用衍生的切割的扩增多态性序列方法来开发检测给定密码子的变化的分子工具，其性质是未知的。我们将它们施加到Alopecurus myosuroides（黑草）中，以形成靶向ALS密码子A122，P197，A205，W574和S653对除草剂敏感性至关重要的测定。这些测定检测到W574L或P197T，或两种取代，在大多数从Als抑制剂在3年后失败的领域分析的大多数植物中。可以容易地设置类似的测定以用于任何物种。鉴于选择对Als抑制剂的抗性的快速度，这些测定应该非常有用于积极的除草剂抗性诊断。

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C DÉLYE; K BOUCANSAUD;
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年度 2008
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正文语种 eng
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1. ALOMYbase, a resource to investigate non-target-site-based resistance to herbicides inhibiting acetolactate-synthase (ALS) in the major grass weed Alopecurus myosuroides (black-grass) [J] . Jeanne Aude Christiane Gardin, Jér?me Gouzy, Sébastien Carrère, BMC Genomics . 2015,第1期

机译：ALOMYbase，一种资源，可用于研究主要草杂草Aurocurus myosuroides（黑草）中非目标部位对除草剂抑制乙酰乳酸合酶（ALS）的抗性
2. Complex genetic control of non-target-site-based resistance to herbicides inhibiting acetyl-coenzyme A carboxylase and acetolactate-synthase in Alopecurus myosuroides Huds [J] . Petit Ccbcile, Duhieu Bastien, Boucansaud Karelle, Plant Science: An International Journal of Experimental Plant Biology . 2010,第6期

机译：基于非目标位点的除草剂抑制乙酰辅酶A羧化酶和乙酰乳酸合酶在除草剂中的复杂遗传控制
3. Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum [J] . Preston C., Powles SB. Heredity: An International Journal of Genetics . 2002,第Pta1期

机译：杂草中除草剂抗性的演变：基于目标位点的硬黑麦草对乙酰乳酸合酶抑制性除草剂的抗性的初始频率
4. Resistance to acetolactate synthase (ALS) inhibiting herbicides in UK populations of the grass-weed Alopecurm myosuroides (black-grass) [C] . R Marshall, S R Moss International Plant Protection Congress . 2007

机译：抗丙酮酸合成酶（ALS）抑制草杂草菌肌室（黑草）的英国群体中的除草剂
5. Resistance to acetolactate synthase-inhibiting herbicides in shattercane and johnsongrass: Current status and future predictions. [D] . Werle, Rodrigo. 2016

机译：对碎甘蔗和约翰逊草中乙酰乳酸合酶抑制除草剂的抗性：现状和未来预测。
6. ALOMYbase a resource to investigate non-target-site-based resistance to herbicides inhibiting acetolactate-synthase (ALS) in the major grass weed Alopecurus myosuroides (black-grass) [O] . Jeanne Aude Christiane Gardin, Jérôme Gouzy, Sébastien Carrère, 2015

机译：ALOMYbase一种资源用于研究主要草杂草Aurocurus myosuroides（黑草）中非目标部位对除草剂抑制乙酰乳酸合酶（ALS）的抗性
7. ALOMYbase, a resource to investigate non-target-site-based resistance to herbicides inhibiting acetolactate-synthase (ALS) in the major grass weed Alopecurus myosuroides (black-grass) [O] . Gardin, Jeanne Aude Christiane, Gouzy, Jérôme, Carrère, Sébastien 2015

机译：ALOMYbase，一种资源，可用于研究主要草杂草Aurocurus myosuroides（黑草）中非目标部位对除草剂抑制乙酰乳酸合酶（ALS）的抗性

A molecular assay for the proactive detection of target site-based resistance to herbicides inhibiting acetolactate synthase in Alopecurus myosuroides

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