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Protein Crystal Growth in the Advanced Protein Crystallization Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alcohol Dehydrogenase Crystals Grown on the Ground and in Microgravity

机译：LMS任务中高级蛋白质结晶设施中的蛋白质晶体生长：在地面和微重力下生长的Sulfolobus solfataricus酒精脱氢酶晶体的比较

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Crystals of alcohol dehydrogenase from Sulfolobus solfataricus were grown in the Advanced Protein Crystallization Facility during the Life and Microgravity Sciences Spacelab mission on the US Space Shuttle. Large diffracting crystals were obtained by dialysis, whereas only poor-quality crystals were obtained by vapour diffusion. The quality of both the microgravity and ground-based crystals was analysed by X-ray diffraction. There was some improvement in terms of size and diffraction resolution limit for the microgravity crystals. However, the twinning observed in the Earth-grown crystals was also present for those grown in microgravity.

机译：在美国航天飞机执行“生命和微重力科学”太空实验室任务期间，在先进的蛋白质结晶设施中生长了来自硫铃虫的乙醇脱氢酶晶体。通过透析获得大的衍射晶体，而通过蒸汽扩散仅获得劣质晶体。通过X射线衍射分析了微重力晶体和地基晶体的质量。在微重力晶体的尺寸和衍射分辨率极限方面有一些改进。然而，对于那些在微重力中生长的晶体，也存在在地球生长的晶体中观察到的孪生现象。

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Esposito L.; Sica F.; Zagari A.; Sorrentino G.; Berisio R.; Carotenuto L.; Giordano A.; Raia C. A.; Rossi M.; Lamzin V. S.; Wilson K. S.;
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年度 1998
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正文语种 eng
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机译：STS-95航天飞机任务中先进蛋白质结晶设施中蛋白质晶体生长的视频观察
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机译：一种可观察的蛋白质晶体生长设备，用于研究微重力对蛋白质结晶的影响
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机译：嗜热古细菌Sulfolobus solfataricus中GTP结合蛋白的纯化结晶和初步晶体学分析
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机译：嗜热古细菌Sulfolobus solfataricus GTP结合蛋白的纯化，结晶和初步晶体学分析
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机译：自动化高通量蛋白质结晶筛选乳酸脱氢酶的非比例和蛋白质结构研究

Protein Crystal Growth in the Advanced Protein Crystallization Facility on the LMS Mission: a Comparison of Sulfolobus solfataricus Alcohol Dehydrogenase Crystals Grown on the Ground and in Microgravity

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