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In vivo and In Vitro Measurements of Ruminal Redox Potential : a Comparative Study

机译：体内和体外测定瘤胃氧化还原电位的比较研究

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This experiment compared ruminal in vivo and in vitro conditions in which redox potential (Eh) and fermentative parameters were measured during 3 consecutive days. A rumen fistulated dry dairy cow was adapted during 13 days to a hay-based diet supplemented with 43% of concentrates. Ruminal pH and Eh were measured in vivo from feeding (0h) to 6 hours (6h) at 15 min interval on d1 and d2. On d3, ruminal fluid was sucked out and divided in 10 flasks for in vitro use. In each flask, substrates (starch, hay and urea) and a buffer solution (pH 7) were added and flasks were kept from light and air at 39° C in a waterbath rotary shaker. The pH and Eh were recorded at the start of incubation (0h) to 6 hours (6h) every 15 min. For both methods, VFA and DL-lactate contents were determined at 0h and 6h. At 0h, in vivo Eh (– 217 mV) differed (P = 0.003) from in vitro value (– 123 mV) probably because of ruminal fluid contact with air outside the rumen. After 45 min, Eh measured in rumen (– 227 mV) were not different from Eh recorded in incubated milieu (– 183 mV). After 2 h, both methods yielded similar Eh values. At 0h, total VFA and DL-lactate contents were significantly different between in vivo (60.1 and 0.03 mM, respectively) and in vitro (36.9 and 0.62 mM, respectively) methodologies, owing to the transfer of rumen fluid and the dilution by buffer for incubation purposes. At 6h, no more significant difference was observed, suggesting therefore that in vitro reflected in vivo conditions. At 6h, contents of individual VFA did not differ (49.1 mM of acetate, 10.4 mM of propionate and 9.16 mM of butyrate, on average). In conclusion, during a 6-h incubation, our in vitro experimental method offered a fermentative and reducing environment close to the rumen. Moreover, this present study put forward the capacity of ruminal microbiota to restore reducing conditions in vitro after an exogenous perturbation.
机译：该实验比较了瘤胃的体内和体外条件，其中连续3天测量了氧化还原电位（Eh）和发酵参数。在13天内，将瘤胃瘘干燥的奶牛适应了以干草为基础的饮食，并补充了43％的浓缩物。在d1和d2上以15分钟的间隔进食（0h）至6小时（6h），在体内测量了瘤胃的pH和Eh。在第3天，将瘤胃液吸出并分成10个烧瓶以供体外使用。在每个烧瓶中，添加底物（淀粉，干草和尿素）和缓冲溶液（pH 7），并在水浴旋转摇床中于39°C保持烧瓶避光和通风。在孵育开始（0h）到每15分钟6小时（6h）记录一次pH和Eh。对于这两种方法，分别在0h和6h时测定VFA和DL-乳酸含量。在0h时，体内Eh（– 217 mV）与体外值（– 123 mV）不同（P = 0.003），可能是因为瘤胃液与瘤胃外的空气接触。 45分钟后，瘤胃中的Eh（– 227 mV）与培养的环境中的Eh（– 183 mV）没什么不同。 2小时后，两种方法均产生相似的Eh值。在0h时，由于瘤胃液的转移和缓冲液的稀释作用，体内（分别为60.1和0.03 mM）和体外（分别为36.9和0.62 mM）方法的VFA和DL-乳酸总含量显着不同。孵化的目的。在6h，没有观察到更多的显着差异，因此表明体外反映了体内条件。在6小时时，各个VFA的含量没有差异（平均为49.1 mM的乙酸盐，10.4 mM的丙酸盐和9.16 mM的丁酸盐）。总之，在6小时的温育过程中，我们的体外实验方法提供了接近瘤胃的发酵和还原性环境。此外，本研究提出了瘤胃微生物群在外源性扰动后在体外恢复还原条件的能力。

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