首页> 外文OA文献 >Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis

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Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis

机译：衣藻DC2的66 kDa同源物（臂对接复合体的一个亚基）与海鞘Ciona intestinalis中的精子鞭毛的Dynein外臂相关

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We previously identified a 66 kDa axonemal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis. Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chlamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2+ in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm.

机译：我们先前在海鞘Ciona intestinalis的精子中鉴定出66 kDa的轴索蛋白（Ci-Axp66.0）。在这里，我们发现Ci-Axp66.0与衣藻衣藻外臂对接复合物的DC2亚基显示序列相似性。对Ci-Axp66.0二级结构的分析表明，与衣藻DC2一样，该分子的N末端三分之二富含卷曲的螺旋结构。免疫金定位显示它位于外臂动力蛋白附近。 Ci-Axp66.0部分通过高盐溶液从轴蛋白中提取，并与外臂动力蛋白共纯化。该共纯化不受隔离缓冲液中Mg2 +的缺乏的影响，表明Ci-Axp66.0与外臂动力蛋白有关。这些结果表明，Ci-Axp66.0是Ciona精子轴突中外臂动力蛋白对接复合物的组成部分。

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Ushimaru Yuji; Konno Aru; Kaizu Maiko; Ogawa Kazuo; Satoh Nori; Inaba Kazuo;
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年度 2006
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1. Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis [J] . Ushimaru Y, Konno A, Kaizu M, Zoological Science . 2006,第8期

机译：衣藻对接复合体亚基衣藻DC2的一个66 kDa同源物与海鞘Ciona intestinalis的精子鞭毛的外在Dynein
2. Transport and arrangement of the outer-dynein-arm docking complex in the flagella of Chlamydomonas mutants that lack outer dynein arms. [J] . Wakabayashi- K, Takada S, Witman GB, Cell motility and the cytoskeleton . 2001,第4期

机译：缺乏外部动力蛋白臂的衣藻突变体鞭毛中的外部动力蛋白臂对接复合体的运输和排列。
3. Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2. [J] . Hozumi A, Satouh Y, Makino Y, Cell motility and the cytoskeleton . 2006,第10期

机译：Ciona精子外臂动力蛋白的分子表征揭示了与外臂对接复合蛋白2相关的多个成分。
4. Structural Analysis of the I1 Inner Dynein Arm Complex from Chlamydomonas Flagella [C] . T. Heuser, C.F. Barber, J. Lin, Microscopy and microanalysis.;Microscopy and microanalysis. . -1

机译：衣藻鞭毛I1内达因臂复合物的结构分析
5. DC3 the 21-kDa Subunit of the Outer Dynein Arm-Docking Complex (ODA-DC) Is a Novel EF-Hand Protein Important for Assembly of Both the Outer Arm and the ODA-DC [O] . Diane M. Casey, Kazuo Inaba, Gregory J. Pazour, 2003

机译：DC3是Dynein臂对接复合物（ODA-DC）的21 kDa亚基是一种新型的EF手蛋白质对于组装外臂和ODA-DC都很重要
6. Association of a 66 kDa homolog of Chlamydomonas DC2, a subunit of the outer arm docking complex, with outer arm dynein of sperm flagella in the ascidian Ciona intestinalis [O] . Ushimaru, Yuji, Konno, Aru, Kaizu, Maiko, 2006

机译：衣藻Chockydomonas DC2的66 kDa同源物，其是外臂对接复合体的一个亚基，与海鞘Ciona肠道中的精子鞭毛的外臂动力蛋白有关

Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis

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