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首页> 外文期刊>Cell motility and the cytoskeleton >Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2.
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Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2.

机译：Ciona精子外臂动力蛋白的分子表征揭示了与外臂对接复合蛋白2相关的多个成分。

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摘要

Using proteomic and immunochemical techniques, we have identified the light and intermediate chains (IC) of outer arm dynein from sperm axonemes of the ascidian Ciona intestinalis. Ciona outer arm dynein contains six light chains (LC) including a leucine-rich repeat protein, Tctex1- and Tctex2-related proteins, a protein similar to Drosophila roadblock and two components related to Chlamydomonas LC8. No LC with thioredoxin domains is included in Ciona outer arm dynein. Among the five ICs in Ciona, three are orthologs of those in sea urchin dynein: two are WD-repeat proteins and the third one, unique to metazoan sperm flagella, contains both thioredoxin and nucleoside diphosphate kinase modules. The remaining two Ciona ICs have extensive coiled coil structure and show sequence similarity to outer arm dynein docking complex protein 2 (DC2) that was first identified in Chlamydomonas flagella. We recently identified a third DC2-like protein with coiled coil structure, Ci-Axp66.0 that is also associated in substoichiometric amounts with Ciona outer arm dynein. In addition, Oda5p, a component of an additional complex required for assembly of outer arm dynein in Chlamydomonas flagella, also groups with this family of DC2-like proteins. Thus, the assembly of outer arm dynein onto doublet microtubules involves multiple coiled-coil proteins related to DC2.
机译：使用蛋白质组学和免疫化学技术，我们从海鞘Ciona intestinalis的精子轴突中鉴定了外臂动力蛋白的轻链和中间链（IC）。 Ciona外臂动力蛋白含有6条轻链（LC），其中包括富含亮氨酸的重复蛋白，Tctex1和Tctex2相关蛋白，类似于果蝇阻滞剂的蛋白以及与衣藻LC8相关的两个成分。 Ciona外臂动力蛋白中不包含具有硫氧还蛋白结构域的LC。在Ciona的5种IC中，有3种与海胆动力蛋白的直系同源物：2种是WD重复蛋白，第3种是后生精子鞭毛特有的，同时含有硫氧还蛋白和核苷二磷酸激酶模块。其余两个Ciona IC具有广泛的盘绕线圈结构，并显示出与首次在衣藻鞭毛中鉴定的外臂动力蛋白对接复合蛋白2（DC2）相似的序列。我们最近发现了第三种具有盘绕线圈结构的DC2样蛋白Ci-Axp66.0，该蛋白也与亚化学计量的Ciona外臂动力蛋白相关。此外，Oda5p是鞭毛衣藻中组装外臂动力蛋白所需的其他复合物的组成部分，也与该类DC2样蛋白一起分组。因此，外臂动力蛋白在双峰微管上的组装涉及与DC2相关的多个卷曲螺旋蛋白。

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