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Label-Free Analysis and Sorting of Microalgae and Cyanobacteria in Microdroplets by Intrinsic Chlorophyll Fluorescence for the Identification of Fast Growing Strains

机译:利用内在叶绿素荧光对小滴中微藻和蓝细菌的无标记分析和分类,以鉴定快速生长的菌株

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摘要

Microalgae and cyanobacteria are promising organisms for sustainable biofuel production, but several challenges remain to make this economically viable, including identification of optimized strains with high biomass productivity. Here we report on a novel methodology for the label-free screening and sorting of cyanobacteria and microalgae in a microdroplet platform. We show for the first time that chlorophyll fluorescence can be used to measure differences in biomass between populations of picoliter microdroplets containing different species of cyanobacteria, Synechocystis PCC 6803 and Synechococcus PCC 7002, which exhibit different growth dynamics in bulk culture. The potential and robustness of this label-free screening approach is further demonstrated by the screening and sorting of cells of the green alga Chlamydomonas reinhardtii encapsulated in droplets.
机译:微藻和蓝细菌是可持续生物燃料生产的有前途的生物,但要使其在经济上可行,仍存在若干挑战,包括鉴定具有高生物量生产率的优化菌株。在这里,我们报告了一种新颖的方法,用于在微滴平台中进行无标签筛选和分类蓝藻和微藻类。我们首次展示了叶绿素荧光可用于测量包含不同种类的蓝细菌,蓝藻PCC 6803和聚球藻PCC 7002的皮升微滴种群之间生物量的差异,它们在散装培养中表现出不同的生长动力学。这种无标记筛选方法的潜力和鲁棒性通过封装在液滴中的绿藻莱茵衣藻细胞的筛选和分选得到进一步证明。

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