首页> 外文OA文献 >PENINGKATAN KUALITAS GIZI DAN DETOKSIFIKASI MIMOSIN TEPUNG DAUN LAMTORO (Leucaena leucocephala) YANG DIFERMENTASI DENGAN Bacillus Sp DAN Trichoderma viride SERTA PENGARUHNYA PADA PRODUKTIVITAS DAN KUALITAS TELUR ITIK PITALAHudud
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PENINGKATAN KUALITAS GIZI DAN DETOKSIFIKASI MIMOSIN TEPUNG DAUN LAMTORO (Leucaena leucocephala) YANG DIFERMENTASI DENGAN Bacillus Sp DAN Trichoderma viride SERTA PENGARUHNYA PADA PRODUKTIVITAS DAN KUALITAS TELUR ITIK PITALAHudud

机译:芽孢杆菌和木霉菌发酵的乳酸菌米粉(Leucaena leucocephala)的营养质量和解毒性能的提高,以及芽孢杆菌和木霉菌发酵量对产卵的影响以及ITB卵生率的提高 ud

摘要

udThe aims of study to know the optimum fermentation conditions , to improve the quality of nutrition and its effect on the detoxification of mimosin fermentation products with Bacillus Sp and Trichoderma viride on the productivity and quality of duck eggs Pitalah. Research experiment was divided into four steps : The first step was isolation, selection and identification of cellulolitic bacteria from digestic gastrointestinal of Pitalah duck. The second step was determination of the optimum conditions for microbial growth and the best for media inoculums. The third step was determination optimum condition for lamtoro leaf meal and the activity cellulase and protease enzyme from Bacillus laterosporus and selluase enzyme from Trichoderma viride based on nutrient quality and quantity of these fermented products. And the forth step was the feeding trial on Pitalah ducks.udResults of the first step showed that these isolates were Bacillus coagulans andudBacillus laterosporus base on biochemical and morphological test. Based on the the higher sellulase activity and wider cleare zone Bacillus laterosporus was selected for the next. In the second step , the best growing time, pH and temperature were detected within 18 hours inculation, 6 and 370C for Bacillus laterosporus respectively. Rice brain was the bestudinoculums media compared to the other meals (corn, tapioca and lamtoro leaf meal) for and Bacillus laterosporus on the colony number 60,5 x 109 CFU/g. In the third step, optimum conditions of the fermentation of lamtoro leaf meal was at 6% dosage of inoculums, 24 hours of fermentation length , The highest activity protease enzyme was 2,682 Unit/ml and cellulaseudenzyme was18,576 Units/ml. Metabilism of Energy 2524,74 kcal/kg, N retention 68,99% and digestibility of crude fiber 57,91; reduction of mimosine 64, 89% and the increasing of B- carotene 96,91% for Bacillus laterosporus. The optimum conditions of the fermentation of lamtoro leaf meal was at 7% dosage of inoculums, 7 days of fermentation length , The highest activity cellulase enzyme was 7,619 Units/ml. Metabolism of Energy 2504,34 kcal/kg, N retention 68,77% and digestibility of crude fiber 58,62; reduction of mimosine 77,ud48% and the increasing of B-carotene 68,96% For fermentation with Trichoderma viride.udThe final step, the using of 20% lamtoro leaf meal fermentation products withudBacillus laterosporus (R3) and product mix Bacillus laterosporus and Trichoderma viride (R6) can generate income over feed costs higher (Rp. 717.39 and Rp. 732.80) and feed conversion (R6) 3, 58 better than the other rations.udIn conclution, lamtoro leaf meal fermented with Bacillus laterosporus andudTrichoderma, and a mix of both can be used up to the level of 20% in the ration of Pitalah ducks.ud
机译:ud研究目的是了解最佳发酵条件,以改善营养质量及其对Bacillus Sp和Trichoderma viride含羞草发酵产物的解毒作用对鸭卵Pitalah生产力和质量的影响。研究实验分为四个步骤:第一步是分离,选择和鉴定来自Pitalah鸭消化胃肠中的纤维素细菌。第二步是确定微生物生长的最佳条件和培养基接种的最佳条件。第三步是根据这些发酵产物的营养质量和数量,确定最佳的lamoro叶粉条件,以及来自芽孢杆菌的活性纤维素酶和蛋白酶以及来自木霉的木脂酶的活性。 ud第一步的结果显示,根据生化和形态学分析,这些分离株为凝结芽孢杆菌和 uderBacilluslateosporus。基于较高的纤维素酶活性和较宽的清除区,下一步选择了芽孢杆菌。在第二步中,在接种后的18小时内检测到最佳的生长时间,pH和温度,分别为稍后的芽孢杆菌和6℃和370℃。与其他餐点(玉米,木薯粉和lamoro叶粉)相比,稻脑是最好的 udinoculums培养基,其菌落数为60,5 x 109 CFU / g。第三步,最佳发酵条件为:接种量为6%,发酵时间为24小时,活性最高的蛋白酶为2,682单位/毫升,纤维素酶/辅酶为18,576单位/毫升。能量新陈代谢2524.74 kcal / kg,氮保留68.99%,粗纤维的消化率57.91;枯草芽孢杆菌减少了64%的含羞草89%,β-胡萝卜素增加了96.91%。最佳发酵条件为接种量为7%,发酵时间为7天,纤维素酶的最高活性为7,619单位/ ml。能量的代谢为2504,34 kcal / kg,N保留率为68.77%,粗纤维的消化率为58,62;减少含羞草碱77% ud48%,增加B-胡萝卜素68.96%用于用木霉菌发酵。 ud最后一步,使用含20%lamtoro叶粉发酵产物的 udBacillus Laterosporus(R3)和产品混合物枯草芽孢杆菌和里氏木霉(R6)可以比饲料成本更高(Rp。717.39和Rp。732.80)和饲料转化率(R6)3、58比其他配比更好地产生收入。和 ud木霉属,并且两者的混合使用量可以达到Pitalah鸭的定量的20%。

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    Nita Yessirita;

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