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Characterization of fruit products by capillary zone electrophoresis and liquid chromatography using the compositional profiles of polyphenols. Application to authentication of natural extracts

机译：使用多酚的组成图谱通过毛细管区带电泳和液相色谱表征水果产品。应用于天然提取物的认证

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Capillary zone electrophoresis (CZE) and high performance liquid chromatography (HPLC) were applied to the authentication of fruit products based on the compositional profiles of polyphenols. Various sample treatments were used to maximize the overall recovery of polyphenols or specific fractions, such as phenolic acids or anthocyanins. The resulting CZE and HPLC data were treated with Principal Component Analysis (PCA) showing that samples were mainly clustered according to the fruit of origin, with cranberry- and grape-based products clearly separated in groups. A possible adulterated cranberry extract was analyzed more deeply by high resolution mass spectrometry (HRMS) in order to identify the presence of A-type proanthocyanidins, which are characteristic and more abundant in cranberry-based products. In accordance with PCA interpretation, HRMS results indicated that the suspicious sample was not a cranberry-based product, allowing us to validate and demonstrate the suitability of both CZE- and HPLC-proposed methods for the characterization of fruit-based products.
机译：基于多酚的组成特征，将毛细管区带电泳（CZE）和高效液相色谱（HPLC）用于水果产品的鉴定。使用了各种样品处理方法，以使多酚或特定馏分（例如酚酸或花青素）的总体回收率最大化。使用主成分分析（PCA）对所得的CZE和HPLC数据进行处理，结果表明样品主要根据产地的果实进行聚类，而基于蔓越莓和葡萄的产品清楚地分为几类。为了识别可能存在的掺假蔓越莓提取物，通过高分辨率质谱（HRMS）进行了更深入的分析，以确定存在于蔓越莓类产品中的特征性且含量较高的A型原花色素。根据PCA的解释，HRMS结果表明可疑样品不是基于蔓越莓的产品，这使我们能够验证和证明CZE和HPLC提出的方法对基于水果的产品进行表征的适用性。

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