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Toward mid-infrared, subdiffraction, spectral-mapping of human cells and tissue: SNIM (scanning near-field infrared microscopy) tip fabrication

机译:迈向人体细胞和组织的中红外,亚衍射,光谱映射:SNIM(扫描近场红外显微镜)尖端制造

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摘要

Scanning near-field infrared microscopy (SNIM) potentially enables subdiffraction, broadband mid-infrared (MIR:3–25-μm wavelength range) spectral-mapping of human cells and tissue for real-time molecular sensing, with prospective use in disease diagnosis. SNIM requires an MIR-transmitting tip of small aperture for photon collection. Here, chalcogenide-glass optical fibers are reproducibly tapered at one end to form a MIR transmitting tip for SNIM. A wet-etching method is used to form the tip. The tapering sides of the tip are Al-coated. These Al-coated tapered-tips exhibit near-field power-confinement when acting either as the launch-end or exit-end of the MIR optical fiber. We report first time optimal cleaving of the end of the tapered tip using focused ion beam milling. A flat aperture is produced at the end of the tip, which is orthogonal to the fiber-axis and of controlled diameter. A FIB-cleaved aperture is used to collect MIR spectra of cells mounted on a transflection plate, under illumination of a synchrotron- generated wideband MIR beam.
机译:扫描近场红外显微镜(SNIM)可能实现亚衍射,人细胞和组织的宽带中红外光谱(MIR:3–25μm波长范围)光谱映射,用于实时分子感测,有望在疾病诊断中使用。 SNIM需要一个小孔径的MIR传输尖端,用于光子收集。在此,硫属化物-玻璃光纤的一端可重复地变细,以形成用于SNIM的MIR透射尖端。使用湿蚀刻法形成尖端。尖端的锥形侧面镀有铝。当用作MIR光纤的出射端或出射端时,这些涂有Al的锥形尖端显示出近场功率限制。我们使用聚焦离子束铣削技术首次报告了锥形尖端末端的最佳劈裂。在尖端的末端产生一个平的孔,该孔垂直于纤维轴并具有受控的直径。 FIB切割的孔径用于在同步加速器产生的宽带MIR光束的照射下收集安装在半透板上的细胞的MIR光谱。

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