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Synthetic seed production and physio-biochemical studies in Cassia angustifolia Vahl. — a medicinal plant

机译：决明子（Cassia angustifolia Vahl）的合成种子生产和生理生化研究。 —药用植物

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Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl · 2HO were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1–6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (P), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.
机译：合成种子技术是传统微繁殖技术的替代产品，可用于生产和递送克隆的幼苗。通过在海藻酸钙凝胶中包囊杜鹃花的节段来生产合成种子。发现3％（w / v）海藻酸钠和100 mM CaCl·2HO最适合包裹节段。在半强度Murashige和Skoog培养基中补充了噻唑隆（5.0μM）+吲哚-3-乙酸（1.0μM）的合成种子在培养8周后表现出最大的芽数（10.9±0.78），表现出体外（78％）转化响应。封装的节段显示了在4°C的不同冷藏期（1-6周）后成功再生。将合成种子在4°C下保存4周，当放回再生培养基后8周后，转化率最高（93％）。在补充有1.0μM吲哚-3-丁酸（IBA）的半强度Murashige和Skoog培养基上培养时，分离出的嫩芽产生了健康的根和生长良好的嫩芽，并且根在装有无菌土的塑料盆中成功硬化。生长室，并逐渐转移到温室，在那里它们生长良好，成活率为85％。将2个月大的体外培育植物的生长性能与相同年龄的体内幼苗进行了比较。桔小球藻光合色素含量，净光合速率（P），超氧化物歧化酶和过氧化氢酶活性的变化表明微繁殖植物适应离体条件。

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