In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 嬠min-1 with a purity of [similar]99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min-1 and a massive throughput of 7 נ108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.
展开▼
机译：在本文中，我们报告了一种具有蛇形通道的简单惯性微流体装置的发展，该装置可有效地从血浆中分离血细胞。该装置的工作机制依赖于蛇形通道中颗粒的两侧二次流动辅助惯性聚焦。具体而言，血细胞沿通道的两侧聚集，而血浆则收集在通道中心内的无细胞区域。用稀释的（1/20）全血测试了该设备。在单个过程中获得了350嬠min-1的相对较高的流速，纯度约为99.75％。经过第二步处理后，纯度进一步提高到99.95％。八个平行的蛇形通道的平行化实现了2.8 ml min-1的高流速和每分钟7×108个细胞的大量通量。我们的设备可以轻松地与其他样品制备过程或检测单元集成在一起，以形成样品到答案的芯片实验室系统。
展开▼
