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Preparation and characterisation of plga-coated porous bioactive glass-ceramic scaffolds for subchondral bone tissue engineering

机译:软骨下骨组织工程用plga涂层多孔生物活性玻璃陶瓷支架的制备与表征

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摘要

The present work involved the development of a poly(lactic-co-glycolic acid) (PLGA)-coated, porous 58S bioactive glass-ceramic scaffold for the bone-forming layer of a future bilayered scaffold for osteochondral tissue engineering. Scanning electron microscopy (SEM), mechanical testing and preliminary cell culture investigations were performed to determine the suitability of the scaffold for the subchondral bone tissue engineering application. The bioactive-glass ceramic scaffolds possessed excellent pore interconnectivity. Mechanical testing of the PLGA-coated bioactive glass-ceramic scaffolds demonstrated a compressive strength of 0.25±0.05 MPa. The capacity of the bioactive glass-ceramic scaffold to proliferate human bone marrow stromal cells (BMSCs) was also assessed through in vitro culturing. SEM morphology investigations revealed that the BMSCs had a relatively high affinity for the PLGA-coated bioactive glass-ceramic scaffold and significant attachment and proliferation towards confluence occurred over a 21 day period.
机译:目前的工作涉及为未来的骨软骨组织工程双层支架的骨形成层开发一种聚乳酸-乙醇酸(PLGA)涂层的多孔58S生物活性玻璃陶瓷支架。进行扫描电子显微镜(SEM),机械测试和初步的细胞培养研究,以确定该支架对软骨下骨组织工程应用的适用性。生物活性玻璃陶瓷支架具有优异的孔互连性。涂覆PLGA的生物活性玻璃陶瓷支架的机械测试表明其抗压强度为0.25±0.05 MPa。还通过体外培养评估了生物活性玻璃陶瓷支架增殖人骨髓基质细胞(BMSCs)的能力。 SEM形态学研究表明,BMSCs对PLGA包被的生物活性玻璃陶瓷支架具有相对较高的亲和力,并且在21天的时间内发生了明显的附着和向融合的扩散。

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