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首页> 外文OA文献 >Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.
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Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.

机译：用DNA进行初免-加强免疫，然后用表达P50的重组牛痘病毒诱导抗犬巴贝斯球菌的保护性免疫。

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摘要

A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization regime induced a specific IgG2 antibody response and IFN-gamma production in dogs. Two weeks after the booster immunization with a recombinant vaccinia virus expressing P50, the dogs were challenged with B. gibsoni patasites. The dogs immunized with the prime-boost regime showed partial protection, manifested as a significantly low level of parasitemia and a 2-day delay of the peak parasitemia. These results indicated that such a heterologous prime-boost immunization approach might be useful against B. gibsoni infection in dogs.
机译：已经显示了用引物DNA，然后是表达相关抗原的重组牛痘病毒的异源初免-加强免疫方案，可以诱导针对几种传染性病原体的有效免疫反应。在这项研究中，我们构建了一个重组质粒和牛痘病毒，两者均表达巴贝斯希氏小球藻的P50，以研究异源初免-加强免疫对犬幼虫的免疫原性和保护功效。与对照组相比，用初免-加强免疫方案免疫的狗产生了显着高水平的针对P50的特异性抗体，并且在用重组牛痘病毒加强免疫后，抗体水平大大提高。初免-加强免疫方案在狗中诱导了特异性IgG2抗体反应和IFN-γ产生。用表达P50的重组牛痘病毒加强免疫两周后，用吉布森毕赤酵母（B. gibsoni）寄生虫攻击狗。初免-加强免疫的狗表现出部分保护作用，表现为明显较低的寄生虫病水平和2天的峰值寄生虫病延迟。这些结果表明，这种异源的初免-加强免疫方法可能对犬中的吉氏短杆菌感染有用。

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