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Deactivation of TEM-1 beta-Lactamase investigated by isothermal batch and non-isothermal continuous enzyme membrane reactor methods

机译：等温间歇和非等温连续酶膜反应器方法研究TEM-1β-内酰胺酶的失活

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The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR). The isothermal batch-mode technique was coupled with the three-state Equilibrium Model of enzyme deactivation, while the results of the EMR experiment were fitted to a four-state molten globule model . The two methods both led to the conclusions that the thermal deactivation of TEM-1 β -lactamase does not follow the Lumry-Eyring model and that the Teq of the enzyme (the point at which active and inactive states are present in equal amounts due to thermodynamic equilibrium) is at least 10 °C from the Tm (melting temperature), contrary to the idea that the true temperature optimum of a biocatalyst is necessarily close to the melting temperature.
机译：使用两种实验技术检查了TEM-1β-内酰胺酶的热失活：一系列等温分批测定和在酶膜反应器（EMR）中的单个连续非等温测定。等温分批模式技术与酶失活的三态平衡模型相结合，而EMR实验的结果适合于四态熔融小球模型。两种方法均得出以下结论：TEM-1β-内酰胺酶的热失活未遵循Lumry-Eyring模型，并且该酶的Teq（由于热力学平衡）至少要比Tm（熔融温度）低10°C，这与生物催化剂的最佳实际温度必然接近熔融温度的想法相反。

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