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首页> 外文OA文献 >Diversity of Bacteria Associated with Bursaphelenchus xylophilus and Other Nematodes Isolated from Pinus pinaster Trees with Pine Wilt Disease
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Diversity of Bacteria Associated with Bursaphelenchus xylophilus and Other Nematodes Isolated from Pinus pinaster Trees with Pine Wilt Disease

机译：松材线虫松材线虫松材线虫和其他线虫相关细菌的多样性

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The pinewood nematode (PWN), Bursaphelenchus xylophilus, has been thought to be the only causal agent of pine wiltdisease (PWD), however, since bacteria have been suggested to play a role in PWD, it is important to know the diversity ofthe microbial community associated to it. This study aimed to assess the microbial community associated with B. xylophilusand with other nematodes isolated from pine trees, Pinus pinaster, with PWD from three different affected forest areas inPortugal. One hundred and twenty three bacteria strains were isolated from PWN and other nematodes collected from 14 P.pinaster. The bacteria strains were identified by comparative analysis of the 16S rRNA gene partial sequence. All except oneGram-positive strain (Actinobacteria) belonged to the Gram-negative Beta and Gammaproteobacteria. Most isolatesbelonged to the genus Pseudomonas, Burkholderia and to the family Enterobacteriaceae. Species isolated in higherpercentage were Pseudomonas lutea, Yersinia intermedia and Burkholderia tuberum. The major bacterial populationassociated to the nematodes differed according to the forest area and none of the isolated bacterial species was found in alldifferent forest areas. For each of the sampled areas, 60 to 100% of the isolates produced siderophores and at least 40%produced lipases. The ability to produce siderophores and lipases by most isolates enables these bacteria to have a role inplant physiological response. This research showed a high diversity of the microbial community associated with B. xylophilusand other nematodes isolated from P. pinaster with PWD.
机译：松材线虫（PWN），松材线虫（Bursaphelenchus xylophilus）被认为是松树Wiltdisease（PWD）的唯一病原体，但是，由于已提出细菌在PWD中起作用，因此了解微生物群落的多样性很重要与之相关。这项研究的目的是评估与松材线虫以及从松树，松树和来自葡萄牙三个不同森林地区的PWD分离的其他线虫相关的微生物群落。从PWN和从14个P.pinaster收集的其他线虫中分离出了123个细菌菌株。通过对16S rRNA基因部分序列进行比较分析来鉴定细菌菌株。除一个革兰氏阳性菌株（放线菌）外，所有细菌均属于革兰氏阴性β和γ-变形杆菌。大多数分离株属于假单胞菌属，伯克霍尔德氏菌属和肠杆菌科。较高百分比分离的物种是黄假单胞菌，中间耶尔森菌和结核伯克霍尔德菌。与线虫相关的主要细菌种群根据森林面积而不同，并且在所有不同的森林地区均未发现任何分离的细菌物种。对于每个采样区域，60至100％的分离物产生铁载体，至少40％的产生脂肪酶。大多数分离物产生铁载体和脂肪酶的能力使这些细菌在植物生理反应中发挥作用。这项研究表明，与PWD分离自P. pinaster的木糖双歧杆菌和其他线虫相关的微生物群落具有高度多样性。

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