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用于检测MDCK细胞残留DNA含量的特异性引物和探针及实时荧光定量PCR试剂盒

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摘要

本发明提供用于检测MDCK细胞残留DNA含量的特异性引物和探针，所述特异性引物为下述序列或为下述序列的互补链序列：上游引物MDCK ND‑F：5’‑CATAACCAAAAGGGACGAAC‑3’；下游引物MDCK ND‑R：5’‑TTCCGAGGTTTATAGAGAGTTGT‑3’；所述探针序列为：5’‑ACCTCACTCATTTACGCCCAC‑3’。本发明还提供相应的试剂盒。本发明的试剂盒能够很好的检测MDCK细胞残留DNA含量，灵敏度高，用时短。

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公开/公告号CN113151412A

专利类型发明专利
公开/公告日2021-07-23

原文格式PDF
申请/专利权人兰州百源基因技术有限公司;
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申请/专利号CN202110371867.8
发明设计人车妍;李潇玲;辛倩;李春;杜弢;徐全乐;沈颂东;杨立坤;车团结;
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申请日2021-04-07
分类号C12Q1/6851(20180101);C12N15/11(20060101);
代理机构11768 北京兴智翔达知识产权代理有限公司;
代理人郭卫芹
地址 730000 甘肃省兰州市城关区雁东路102号20层A01-A08
入库时间 2023-06-19 11:59:12

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法律状态公告日

法律状态信息

法律状态
2023-06-16

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1. 用于检测MDCK细胞残留DNA含量的特异性引物和探针及实时荧光定量PCR试剂盒 [P] . 中国专利： CN113151412A . 2021-07-23
2. 用于检测小麦苗枯病菌的特异性引物和探针及实时荧光定量PCR检测试剂盒 [P] . 中国专利： CN113528681A . 2021-10-22
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