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ZELLSTAMM ZUR REDUKTION DER PRODUKTION VON REPRODUZIERBAREM ADENOVIRUS, KONSTRUKTIONSVERFAHREN UND VERWENDUNG

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Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace the gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.

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公开/公告号EP3670650A4

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公开/公告日2020.07.08

原文格式PDF
申请/专利权人 Cansino Biologics Inc.;
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申请/专利号EP18851666
发明设计人 ZHU, Tao;CUI, Haiyan;CHEN, Weiwei;DUAN, Lei;LI, Junqiang;MA, Jin;XIN, Chunlin;SHAO, Zhongqi;YU, Xuefeng;MAO, Helen Huihua;
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申请日2018.08.29
分类号
国家 EP
入库时间 2022-08-21 10:53:50

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