首页>
外国专利>
METHOD OF PRODUCING GENETICALLY MODIFIED LINES OF CELLS OF NATURAL KILLERS WITH KNOCKOUT PD-1 GENE AND HIGH EXPRESSION OF PROTEINS OF FAMILY OF TUMOUR NECROSIS FACTOR FOR IMMUNOTHERAPY OF ONCOLOGICAL DISEASES
METHOD OF PRODUCING GENETICALLY MODIFIED LINES OF CELLS OF NATURAL KILLERS WITH KNOCKOUT PD-1 GENE AND HIGH EXPRESSION OF PROTEINS OF FAMILY OF TUMOUR NECROSIS FACTOR FOR IMMUNOTHERAPY OF ONCOLOGICAL DISEASES
展开▼
机译:基因敲除的PD-1基因和肿瘤坏死因子家族蛋白高表达天然杀伤细胞的遗传修饰细胞系的治疗方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
FIELD: medicine.;SUBSTANCE: this group of inventions refers to medicine and genetic engineering. Disclosed are methods of producing modified killer (NK) cell lines with knockout PD-1 gene or, in other embodiments, with knockout PD-1 gene and constitutive high expression of Fas-ligand, or with knockout PD-1 gene and constitutive high expression of TRAIL. What is also described is a method of selecting modified NK cells with knockout PD-1 gene, which is carried out using a selective zeocin marker. What is also presented is using the obtained cell lines in order to induce apoptosis or lysis of mammalian cancer cells.;EFFECT: present group of inventions provides high output of modified NK cell lines exhibiting high tumour cell growth inhibition activity.;14 cl, 4 dwg
展开▼