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CANOLA HO/LL RESISTANT TO CLUBROOT OF CRUCIFEROUS DISEASE

机译：卡诺拉对猪蹄类病菌的HO / LL具有抗性

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FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology and is a method for identifying a keel-resistant cruciferous plant, including the phenotype of a high content of oleic acid, a low content of linolenic acid (HO/LL), where the content of oleic acid is approximately 77 %, and the content of linolenic acid is approximately 3 %. Method involves the isolation of genomic DNA from keel-resistant cruciferous plants Brassica; screening of isolated genomic DNA for gene fad2, that encodes a polypeptide without delta-12-desaturase oleate activity, and a gene fad3, which encodes a polypeptide without delta-15-desaturase linoleate activity; and identification of cruciferous keel-resistant keel plants when these genes are detected fad2 and fad2 in genomic DNA.;EFFECT: invention provides an efficient method for identifying cruciferous keel-resistant keel plants.;3 cl, 9 dwg, 6 tbl, 8 ex

机译：技术领域本发明涉及生物技术，并且是一种鉴定抗龙骨的十字花科植物的方法，其包括高含量油酸，低含量亚麻酸（HO / LL）的表型，其中油酸的含量约为77％，亚麻酸的含量约为3％。该方法涉及从抗龙骨十字花科植物芸苔中分离基因组DNA。 fad2 基因的分离基因组DNA的筛选，该基因编码不具有delta-12去饱和酶油酸酯活性的多肽，而基因 fad3 编码不具有delta-15-亚油酸去饱和酶活性;基因组DNA中检测到 fad2 和 fad2 的基因鉴定和鉴定十字花科抗龙骨植物;效果：本发明提供了一种鉴定十字花科抗龙骨的有效方法植物;; 3 cl，9 dwg，6 tbl，8 ex 展开▼

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公开/公告号RU2711934C2

专利类型

公开/公告日2020-01-23

原文格式PDF

申请/专利权人 DOW AGROSCIENCES LLC;
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申请/专利号RU20170114351

发明设计人 GINGERA GREGORY R. (CA);ZHAO JIANWEI (CA);RIPLEY VAN LEONARD (CA);UBAYASENA LASANTHA (US);
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申请日2012-09-27

分类号C12N15/87;C12P7/64;C12Q1/68;

国家 RU

入库时间 2022-08-21 11:02:41

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