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Microfluidic chip device for optical force measurement and cell imaging using microfluidic chip construction and mechanics
Microfluidic chip device for optical force measurement and cell imaging using microfluidic chip construction and mechanics
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机译：使用微流体芯片构造和力学的用于光学力测量和细胞成像的微流体芯片装置
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摘要
The present invention relates to a microfluidic chip configuration in which injection occurs in an upward vertical direction, and fluid containers are placed under the chip to minimize particle precipitation in and before the analysis portion of the channels of the chip. In one aspect, the input and fluid flow upward through the bottom of the chip using the manifold, which prevents orthogonal reorientation of the fluid dynamics. The contents of the vial are placed under the chip and pumped directly upwards and vertically into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. In addition, the channel takes a short horizontal turn that almost excludes any influence of cell precipitation due to gravity and zero flow velocity in the walls. The fluid is the highest channel/fluid point on the chip and is thus pumped up to the horizontal analysis portion close to the top of the chip, resulting in sharper imaging. The laser can also suspend cells or particles in this channel during analysis, which prevents their precipitation.
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