METHOD OF PROCESSING LONICERA JAPONICA TO INCREASE ANTI-OXIDANT AND ANTI-OBESITY ACTIVITY

• 1. 含有葫芦科植物提取物或者从其中分离的具有抗脂肪形成和抗肥胖症活性的纯化提取物的组合物 [P] . 中国专利： CN1901926A . 2007-01-24
• 2. 包含抑制二肽基肽酶-IV活性的化合物以及其他抗糖尿病剂或抗肥胖剂作为活性成分的、用于预防和治疗糖尿病或肥胖症的药物组合物 [P] . 中国专利： CN102256605B . 2014.05.07
• 3. METHOD OF PROCESSING LONICERA JAPONICA TO INCREASE ANTI-OXIDANT AND ANTI-OBESITY ACTIVITY [P] . 韩国专利： KR20200056013A . 2020-05-22

  机译： 处理日本忍冬属植物以提高抗氧化剂和抗肥胖活性的方法

• 4. METHOD OF PROCESSING LONICERA JAPONICA THUNB TO INCREASE ANTI-OXIDANT AND ANTI-OBESITY [P] . KR20210044753A . 2021-04-23

  机译： 加工Lonicera japonica Thunb的方法增加抗氧化剂和抗肥胖

• 5. METHOD OF PROCESSING LONICERA JAPONICA THUNB TO INCREASE ANTI-OXIDANT AND ANTI-OBESITY [P] . KR20210045964A . 2021-04-27

  机译： 加工Lonicera japonica Thunb的方法增加抗氧化剂和抗肥胖

• 1. Extraction Process and Content Determination of Total Flavonoids in Lonicera japonica [J] . Yao HUANG, Xiumei MA, Chao ZENG, 药用植物：英文版 . 2020,第006期
• 2. Extraction Process Optimization and Anti-oxidant Activity of Total Flavonoids in Dendrobium nobile Lindl. [J] . Huashan GAO, Minghui CHEN, Guang QI, 药用植物：英文版 . 2020,第004期
• 3. ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF ETHANOL EXTRACT FROM LONICERA JAPONICA THUNB. BY INVOLVING INHIBITING LIPID PEROXIDATION AND INFLAMMATION PROCESS [J] . Wang J. M., Zhao H. P., Cai H., Basic & clinical pharmacology & toxicology. . 2018,第Suppla1期
• 4. Validation of a NIR quantification method for the determination of chlorogenic acid in Lonicera japonica solution in ethanol precipitation process. [J] . Zhisheng Wu, Bing Xu, Min Du, Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis . 2012,第Null期
• 5. S-Sulfocysteine simplifies fed-batch processes and increases the CHO specific productivity via anti-oxidant activity [J] . Hecklau Caroline, Pering Sascha, Seibel Ronja, Journal of Biotechnology . 2016,第Null期
• 6. A METHODOLOGY FOR THE QUANTITATIVE ASSESSMENT OF THE RISK INCREASE IN PROCESS PLANTS DUE TO SEISMIC ACTIVITY [C] . Giacomo Antonioni, Valerio Cozzani, Gigliola Spadoni International Conference on Probabilistic Safety Assessment and Management . 2006
• 7. Comparative ecological and genetic studies between a native (Lonicera sempervirens L.) and an introduced congener (Lonicera japonica Thunb.). [D] . Schierenbeck, Kristina Ann. 1992
• 8. Co-designing organisational improvements and interventions to increase inpatient activity in four stroke units in England: a mixed-methods process evaluation using normalisation process theory [O] . David Clarke, Karolina Gombert-Waldron, Stephanie Honey, 2021
• 9. S-Sulfocysteine simplifies fed-batch processes and increases the CHO specific productivity via anti-oxidant activity [O] . Hecklau Caroline, Pering Sascha, Seibel Ronja, 2016

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Anti-encystment and amoebicidal activity of Lonicera japonica Thunb. and its major constituent Chlorogenic acid in vitro [J] . Tooba Mahboob ,Abdul-Majid Azlan ,Tian-Chye Tan . 亚太热带医药杂志：英文版 . 2016,第9期
• 3. Increase Activity Cavitations in Liquids and Melts at Ultrasonics Processing [J] . Vladimir L. Lanin . 声学期刊（英文） . 2013,第3期
• 4. 几种忍冬属植物中生物活性物质的提取研究 [C] . 孙凌峰 . 第六届全国农副产品综合利用化学学术会议 . 1995
• 5. 五份忍冬属植物农艺性状调查、有效成分与抗氧化活性分析 [A] . 王腊梅 . 2016