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METHOD FOR ESTIMATING CELL COUNT, AND DEVICE FOR ESTIMATING CELL COUNT

机译:估算细胞数的方法及估算细胞数的装置

摘要

[Problem] To provide a method and device for noninvasively estimating a cell count. [Solution] In the present invention, the concentration of a cell metabolite included in a culture medium for culturing certain cells is estimated using a simple method, e.g., using spectroscopy. The cell count of the certain cells can be estimated by fitting to relationship information obtained in advance indicating a relationship between the concentration of the cell metabolite and the cell count of the certain cells. In spectroscopy herein, because the actual concentration of a substance consumed by the cells can be estimated more accurately the higher the concentration of the cell metabolite is, the concentration of a substance consumed by the cells, which decreases over time over the course of culturing of the cells, is estimated in the initial-to-intermediate stage of cell culturing, and the concentration of a cell product which increases over time over the course of culturing of the cells is estimated in the intermediate-to-late stage of cell culturing. The cell count can thereby be estimated through the entire range from the initial stage to the ending of cell culturing.
机译:[问题]提供一种用于非侵入性地估计细胞计数的方法和装置。 [解决方案]在本发明中,用于培养某些细胞的培养基中所包含的细胞代谢物的浓度是通过简单的方法,例如使用光谱法来估计的。可以通过拟合预先获得的指示细胞代谢物的浓度与某些细胞的细胞数之间的关系的关系信息来估计某些细胞的细胞数。在本文的光谱学中,因为细胞代谢产物的浓度越高,则细胞消耗的物质的实际浓度可以被更准确地估计,因此细胞消耗的物质的浓度在培养过程中随时间降低。在细胞培养的初始到中间阶段估计细胞,并且在细胞培养的中晚期估计在细胞培养过程中随时间增加的细胞产物的浓度。因此,可以在从细胞培养的初始阶段到结束的整个范围内估计细胞数。

著录项

  • 公开/公告号WO2020090225A1

    专利类型

  • 公开/公告日2020-05-07

    原文格式PDF

  • 申请/专利权人 PHC HOLDINGS CORPORATION;

    申请/专利号WO2019JP34982

  • 发明设计人 SAKURAI TSUBASA;TAIRA KEISUKE;

    申请日2019-09-05

  • 分类号C12Q1/06;C12M1;

  • 国家 WO

  • 入库时间 2022-08-21 11:11:26

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