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Quantification of nucleosome modifications using chemically-defined recombinant nucleosomes

机译:使用化学定义的重组核小体定量核小体修饰

摘要

The invention relates to the use of recombinant / semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively "modified nucleosomes" or "nucleosome modifications")from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.
机译:本发明涉及带有组蛋白和/或DNA修饰的重组/半合成核小体作为定量共价修饰的(在组蛋白或包裹DNA上),变体或突变核小体(统称为“修饰的核小体”)的参考标准的用途。或“核小体修饰”)。本发明还涉及使用该测定法准确地定量单个或组合的核小体修饰作为疾病的生物标志物的方法。

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