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METHOD FOR GENERATING AN OVERVIEW IMAGE USING A LARGE-APERTURE LENSOBJECTIVE
METHOD FOR GENERATING AN OVERVIEW IMAGE USING A LARGE-APERTURE LENSOBJECTIVE
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机译：利用大口径透镜产生概图的方法
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摘要
The invention relates to a method for generating an overview image using a large-aperture lens objective (4), comprising the following steps: providing an optical detection beam path (2) with the lens objective (4), wherein the detection beam path (2) has a first numerical aperture (NA1) in a first operating state and hasas well as having a first depth of field (ST1) which lies substantially in a focal plane of the detection beam path (2) and, in the first operating state, an image of a detection irradiation with the fist depth of field (ST1) and with a lateral image field is or can be captured, wherein the detection irradiation is affected such that at least one light sheet (16) is directed along an illumination axis (15) into a sample volume (8) to be mapped in thea sample space; transferring the detection beam path (2) into a second operating state, in which the first depth of field (ST1) is increased to a second depth of field (ST2), wherein the large- aperture lens objective (4) remains in the detection beam path (2); and in the second operating state, detecting a detection irradiation, wherein the detection irradiation coming out of a sample space is collected by the lens objective (2), guided along the detection beam path (2) and captured as an image with a lateral image field by at least one detector (7) that is sensitive to the detection irradiation, wherein the lateral image fields are equal in the first and second operating states.
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