首页> 外国专利> PROCESS OF MAGNETIC PARTICLE ENCAPSULATION IN ERYTHROCYTES BASED ON SAGM HYPOTONIC MEDIUM FOR VARIOUS MEDICAL APPLICATIONS

PROCESS OF MAGNETIC PARTICLE ENCAPSULATION IN ERYTHROCYTES BASED ON SAGM HYPOTONIC MEDIUM FOR VARIOUS MEDICAL APPLICATIONS

机译:基于SAGM低渗介质的红细胞中磁性颗粒的包封过程在各种医学中的应用

摘要

The invention relates to a process of magnetic nanoparticles encapsulation in human erythrocytes used as MRI contrast agents. According to the invention, the process consists of erythrocytes dialysis in the presence of iron-based magnetic nanoparticles with sizes of 5...55 nm, suspended in saline solution, for 1...4 h, using 50...75% a SAGM hypotonic medium comprising 150 mM of NaCl, 1.25 mM of adenine, 45 mM of dextrose, 30 mM of mannitol, 2.51 mM of monosodium phosphate, at the temperature of 25°C, while continuously stirring, followed by the recovery of the erythrocytes treated by centrifugation at 600 g and the washing with preservation medium hypertonic solution, to result in human erythrocytes charged with magnetic/superparamagnetic nanoparticles having an encapsulated Fe content of 38.5...69.25%, with an appropriate cell viability, similar to the native erythrocytes.
机译:本发明涉及将磁性纳米颗粒封装在用作MRI造影剂的人红细胞中的方法。根据本发明,该方法由在尺寸为5 ... 55 nm的铁基磁性纳米颗粒存在下透析红细胞,悬浮在盐溶液中1 ... 4 h,使用50 ... 75%在25°C的温度下,在持续搅拌的同时,在150°C的NaGM,150mM的NaCl,1.25mM的腺嘌呤,45mM的葡萄糖,30mM的甘露醇,2.51mM的磷酸二氢钠的SAGM低渗介质中,同时不断回收红细胞通过600 g离心处理,并用保存的高渗溶液洗涤,得到的人红细胞中带有磁性/超顺磁性纳米粒子,其包裹的Fe含量为38.5 ... 69.25%,具有与天然红细胞相似的适当细胞活力。

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