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violacein and deoxyviolacein production process optimized by factorial design and response surface analysis by chromobacterium violaceum

机译：通过析色设计和紫质色杆菌响应面分析优化了紫精素和脱氧紫精素的生产工艺

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摘要

"Violacein and deoxyviolacein production process optimized by factorial design and response surface analysis by chromobacterium violaceum". It is a procedure for optimizing the production of violacein and deoxyviolacein through experimental designs based on statistical principles. In the initial phase 11 factors were analyzed: carbon source (glucose), yeast extract, methionine, vitamin b12, l-tryptophan, agitation, temperature, ph (phosphate buffer), peptone, inoculum and zinc sulfate concentration. a standard fractional experimental design 2 ^ 15-11 ^ was used. In the second phase a central composite planning involving 5 factors: carbon source (glucose), yeast extract, 1-tryptophan, peptone and zinc sulfate. were chosen based on the result of the previous fractional factorial design. The planning consists of a fractional factorial 2 ^ 5 ^. In the final phase of optimization three factors: carbon source (glucose), yeast extract and l-tryptophan were studied using a central three-factor composite design. the average gross violacein production in this schedule was 0.36 g / l. 50% higher than in the other models studied. The average cell mass for this design was 16.59 g / l, around 2.2 times higher than in other models.

机译：“通过因子设计和紫质色杆菌响应面分析，优化了紫精和脱氧紫精的生产工艺”。这是通过基于统计原理的实验设计来优化紫精和脱氧紫精生产的程序。在初始阶段，分析了11个因素：碳源（葡萄糖），酵母提取物，蛋氨酸，维生素b12，l-色氨酸，搅拌，温度，ph（磷酸盐缓冲液），蛋白one，接种物和硫酸锌浓度。使用标准分数实验设计2 ^ 15-11 ^。在第二阶段，中央综合计划涉及五个因素：碳源（葡萄糖），酵母提取物，1-色氨酸，蛋白ept和硫酸锌。根据先前的分数阶乘设计结果选择。该计划由分数阶乘2 ^ 5 ^组成。在优化的最后阶段，使用中央三因素复合设计研究了三个因素：碳源（葡萄糖），酵母提取物和l-色氨酸。在此时间表中，平均紫色素总产量为0.36 g / l。比其他研究模型高出50％。此设计的平均细胞质量为16.59 g / l，约为其他模型的2.2倍。

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公开/公告号BRPI0100199B1

专利类型
公开/公告日2019-11-26

原文格式PDF
申请/专利权人 UNIVERSIDADE ESTADUAL DE CAMPINAS - UNICAMP;
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申请/专利号BR2001PI00199
发明设计人 ARMANDO SEMEDO MENDES;JOÃO ERNESTO DE CARVALHO;MARTA CRISTINA TEIXEIRA DUARTE;NELSON EDUARDO DURÁN CABALLERO;ROY EDWARD BRUNS;
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申请日2001-01-04
分类号A61K35/74;A61P31/04;A61P33;A61P35;
国家 BR
入库时间 2022-08-21 11:18:14

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