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首页> 外国专利> The production process of the violaceu00ecna and deoxiviolaceina optimized by factorial design and analysis of superfu00eccie response by Chromobacterium violaceum

The production process of the violaceu00ecna and deoxiviolaceina optimized by factorial design and analysis of superfu00eccie response by Chromobacterium violaceum

机译：通过因子设计优化分析紫胶和紫胶丝藻的生产工艺，并分析紫胶杆菌对超微藻的反应

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"Production process of violaceu00ecna and deoxiviolaceina optimized by factorial design and response surface analysis of the Chromobacterium violaceum".This is a procedure for the optimization of the production of violaceu00edna and deoxiviolaceu00edna through experimental design based on statistical principles.In the initial phase 11 factors were analyzed: carbon source (glucose), yeast extract, methionine, vitamin B12, L - tryptophan, agitation, temperature, pH (phosphate buffer), peptone,Inoculum and concentration of zinc sulphate. An experimental design necessary standard 2 ^ 15 - 11 ^ was used.In the second phase, a planning central compound involving five factors: carbon source (glucose), yeast extract, peptone and L - tryptophan, zinc sulfate.Were chosen based on results of previous fractional factorial design. The planning consists in a factorial fractional 2 ^ 5 ^.In the final stage of the optimization three factors: carbon source (glucose, yeast extract and L - tryptophan were studied using a central planning composed of three factors.The average production of crude violaceu00edna this planning was 0.36 g / L. some 50% higher than in the other models studied.The mean cell mass for this planning was 16.59 g / L, around 2.2 times higher than in other models.
机译：“通过因子设计和紫罗兰色杆菌的响应面分析优化了紫罗兰和脱氧紫罗兰的生产工艺”。这是通过统计学方法通过实验设计优化紫罗兰和脱氧紫罗兰的生产过程的程序。在初始阶段，分析了11个因素：碳源（葡萄糖），酵母提取物，蛋氨酸，维生素B12，L-色氨酸，搅拌，温度，pH（磷酸盐缓冲液），蛋白one，接种物和硫酸锌浓度。使用实验设计必需的标准品2 ^ 15-11 ^。第二阶段，计划的中心化合物涉及五个因素：碳源（葡萄糖），酵母提取物，蛋白ept和L-色氨酸，硫酸锌。先前的分数阶乘设计。该计划包括一个阶乘分数2 ^ 5 ^。在优化的最后阶段，使用由三个因素组成的中央计划研究了碳源（葡萄糖，酵母提取物和L-色氨酸）三个因素。该计划的重量为0.36 g / L，比其他研究模型高约50％。该计划的平均细胞质量为16.59 g / L，约为其他模型的2.2倍。

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