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METHOD FOR EXTRACTING PHARMACOGENETIC DNA FROM BIOLOGICAL FLUID RETAINED IN A SOLID RESIN COLLECTION DEVICE
METHOD FOR EXTRACTING PHARMACOGENETIC DNA FROM BIOLOGICAL FLUID RETAINED IN A SOLID RESIN COLLECTION DEVICE
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机译：从固体树脂收集装置中保留的生物流体中提取药物基因组DNA的方法
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摘要
A method for extracting pharmacogenetic DNA from dried blood that is retained in a solid resin collection device includes one or more of the steps of combining the dried blood and at least a portion of the solid resin collection device in a first vessel, sonicating the first vessel containing the dried blood and at least a portion of the solid resin collection device, moving at least some of the contents of the first vessel including the portion of the solid resin collection device to a solid resin spin column, adding an elution buffer to the solid resin spin column, centrifuging the solid resin spin column so that at least some of the contents of the solid resin spin column are transferred to a second vessel, and processing the contents of the second vessel by conducting one of capillary electrophoresis, Next-Generation Sequencing, DNA sequencing or genotyping, and mass spectrometry-based sequencing on the contents of the second vessel.
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