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Method for extracting pharmacogenetic DNA from biological fluid retained in a solid resin collection device

机译:从固体树脂收集装置中保留的生物液中提取药物发生DNA的方法

摘要

A method for extracting pharmacogenetic DNA from dried blood that is retained in a solid resin collection device includes one or more of the steps of combining the dried blood and at least a portion of the solid resin collection device in a first vessel, sonicating the first vessel containing the dried blood and at least a portion of the solid resin collection device, moving at least some of the contents of the first vessel including the portion of the solid resin collection device to a solid resin spin column, adding an elution buffer to the solid resin spin column, centrifuging the solid resin spin column so that at least some of the contents of the solid resin spin column are transferred to a second vessel, and processing the contents of the second vessel by conducting one of capillary electrophoresis, Next-Generation Sequencing, DNA sequencing or genotyping, and mass spectrometry-based sequencing on the contents of the second vessel.
机译:一种用于将保留在固体树脂收集装置中的干燥血液中的药物发生DNA的方法包括一个或多个将干燥的血液和至少一部分固体树脂收集装置组合在第一容器中的步骤,超声处理第一容器 含有干燥的血液和至少一部分的固体树脂收集装置,将第一容器的至少一些内容物移动,包括固体树脂收集装置的一部分到固体树脂旋转柱,将洗脱缓冲液加入固体 树脂旋转柱,离心固体树脂旋转塔,使得固体树脂旋转塔的至少一些内容物转移到第二容器中,并通过进行毛细管电泳,下一代测序来处理第二容器的内容物 ,DNA测序或基因分型,以及基于质谱的基于含量的含量的测序。

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