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Transit peptide from Arabidopsis chloroplast ATP sulfurylase 1 and methods of use

机译：拟南芥叶绿体ATP硫化酶1的转运肽及其使用方法

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摘要

The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides. 展开▼

机译：本发明提供了编码融合蛋白的重组DNA分子，其包含与编码客运蛋白的第二DNA序列可操作连接的编码高效转运肽的第一DNA序列，其中所述高效转运肽选自下组：叶绿体40 kD（prTic40），伴侣蛋白10-2（prCpn10-2），原纤维蛋白1B（prFibrillin），ATP硫酸化酶1（prAPS1），ATP硫化酶3（prAPS3）的内膜上的转运子前体的转运肽，5'-腺苷酸硫酸还原酶3（prAPR3），基质抗坏血酸过氧化物酶（prsAPX），prTic40-E2A（prTic40变体），prCpn10-1-ΔC7C37S（伴侣蛋白10-1变体），是任何转运肽的功能片段及其等效物。并且本发明还提供了一种使用高效转运肽将蛋白质高效递送到质体中的方法。展开▼

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公开/公告号US10577621B2

专利类型

公开/公告日2020-03-03

原文格式PDF

申请/专利权人 ACADEMIA SINICA;
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申请/专利号US201816179052

发明设计人 HSOU-MIN LI;CHIUNG-CHIH CHU;
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申请日2018-11-02

分类号C12N15/82;

国家 US

入库时间 2022-08-21 11:27:19

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1. 叶绿体转运肽及其使用方法 [P] . 中国专利： CN103748108A . 2014-04-23

2. 拟南芥FtsHi5基因在调控拟南芥叶绿体发育中的应用 [P] . 中国专利： CN108384794A . 2018-08-10

3. Transit peptide from Arabidopsis chloroplast chaperonin 10-2 and methods of use [P] . 美国专利： US10577620B2 . 2020-03-03

机译： 拟南芥叶绿体伴侣蛋白10-2的转运肽及其使用方法

4. Synthetic Nucleic Acid Molecule derived from Brassica, which encodes a Peptide sequences comprising a First and a Second chloroplast Transit Peptide; encoded Chimeric polypeptide and Method for producing a transgenic Plant. [P] . CL2014002017A1 . 2014-11-21

机译：源自芸苔属的合成核酸分子，其编码包含第一和第二叶绿体转运肽的肽序列;编码的嵌合多肽和产生转基因植物的方法。

5. Chloroplast Transit peptides of the Protein of interest operably linked to Nucleic Acids coding for these peptides Expression Vectors,And Plant material to express these Proteins (Divisional Sun. No 2774 - 14). [P] . CL2018000837A1 . 2018-07-06

机译：目的蛋白的叶绿体转运肽可操作地连接至编码这些肽的核酸表达载体，以及表达这些蛋白质的植物材料（Divisional Sun. No. 2774-14）。

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12. Differential Subcellular Localization and Expression of ATP Sulfurylase and 5′-Adenylylsulfate Reductase during Ontogenesis of Arabidopsis Leaves Indicates That Cytosolic and Plastid Forms of ATP Sulfurylase May Have Specialized Functions [O] . Carmen Rotte, Thomas Leustek 2000

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