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Analytical system for orthogonal access to biomolecules in cell compartments and tagging of biomolecules in cell compartments
Analytical system for orthogonal access to biomolecules in cell compartments and tagging of biomolecules in cell compartments
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机译：正交获取细胞室内生物分子并标记细胞室内生物分子的分析系统
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摘要
The present invention provides a system and method for enhancing access to nuclear information molecules, such as DNA, RNA, and proteins, by a biomolecule for analysis, such as a transposome complex, by treating the nucleus with a nuclear permeability enhancer. And methods of using nuclear membrane, cell membrane and outer compartmentalization approaches as proximity preservation elements. The present invention is, for example, a method of reacting a cell nucleus information molecule with a biomolecule for analysis, the step of contacting the cell nucleus with a nuclear permeability enhancer, and the step of reacting the information molecule with the biomolecule for analysis. A method comprising:
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