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METHODS AND SYSTEMS FOR IN VIVO FULL-FIELD INTERFERENCE MICROSCOPY IMAGING

机译:体内全场干涉显微成像的方法和系统

摘要

According to one aspect, the invention relates to a system (101) for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample. It comprises a full-field OCT imaging system (130) for providing en face images of the sample, wherein said full-field OCT system comprises an interference device (145) with an object arm (147) intended to receive the sample and a reference arm (146) comprising an optical lens (134) and a first reflection surface (133), and an acquisition device (138) configured to acquire a temporal succession of two-dimensional interferometric signals (I1, I2) resulting from interferences produced at each point of an imaging field; an OCT imaging system (110) for providing at the same times of acquisition of said two-dimensional interferometric signals, cross-sectional images of both the sample and a first reflection surface (133) of said full-field OCT imaging system (130); a processing unit (160) configured to determine a plurality of en face images (X - Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals (I1, I2) having a given phase shift; determine from the cross-sectional images provided by the OCT imaging system (110) at the times of acquisition of each of said two two-dimensional interferometric signals (I1, I2) a depth (z) for each en face image (X - Y) of said plurality of slices; determine a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.
机译:根据一个方面,本发明涉及一种用于对散射的三维样本进行体内全视野干涉显微镜成像的系统(101)。它包括用于提供样品的脸图像的全视野OCT成像系统(130),其中,所述全视野OCT系统包括具有目标臂(147)的干涉装置(145)。接收样品和包括光学透镜(134)和第一反射面(133)的参考臂(146),以及配置为获取二维干涉信号的时间序列(I 1 ,I 2 )是由成像场各点产生的干扰引起的; OCT成像系统(110),用于同时获取所述二维干涉信号,样品和所述全场OCT成像系统(130)的第一反射面(133)的横截面图像;处理单元(160),其被配置为确定样本的多个切片的多个脸图像(X-Y),每个 en 图像是从具有给定相移的至少两个二维干涉信号(I 1 ,I 2 );从OCT成像系统(110)提供的横截面图像中确定在获取所述两个二维干涉信号(I 1 ,I 2 )所述多个切片的每个脸图像(X-Y)的深度(z);从所述样本的多个切片和深度的所述多个“脸”图像确定样本的3D图像。

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