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首页> 外国专利> METHOD FOR CONTINUOUSLY CULTURING ASPERGILLUS NIGER SEEDS AND PRODUCING CITRIC ACID USING SAME

METHOD FOR CONTINUOUSLY CULTURING ASPERGILLUS NIGER SEEDS AND PRODUCING CITRIC ACID USING SAME

机译：连续栽培黑曲霉种子并用相同方法生产柠檬酸的方法

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摘要

Disclosed is an Aspergillus niger seed continuous culture method, comprising the steps of: (1) at a startup stage, Aspergillus niger spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture, keeping growth environment of the thallus maintain in an optimal state, and avoiding strain degeneration, so that the seed liquid can be in a continuous and stable high-vitality state, and corresponding fermentation citric acid producing performance can be significantly improved.
机译：本发明公开了一种黑曲霉种子连续培养方法，包括以下步骤：（1）在启动阶段，将黑曲霉孢子接种到种子培养基中得到种子液; （2）在种子连续培养阶段，对步骤（1）中得到的种子液进行连续分散处理，对通过分散得到的种子液进行连续培养，同时补充新鲜的种子进料培养基。 （3）在停止阶段，停止新鲜种子饲料培养基的补充和分散处理，进行连续培养以获得种子液，然后将种子液转移到发酵培养基中进行发酵培养。本发明的方法取得了突破性进展，解决了多细胞丝状细菌生长缓慢，菌丝体在连续培养中容易丢失的问题，从而充分实现了种子的连续培养，使菌体的生长环境保持在最佳状态。避免了菌株的变性，使种子液处于连续稳定的高活力状态，可以显着提高相应的发酵柠檬酸生产性能。

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