A method of obtaining nauplii from artemia cysts in salty water bodies of Kalmykia

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• 3. Method for obtaining free-floating Artemia nauplii from Artemia at the stage of cysts and set for aquaculture [P] . 俄罗斯专利： RU2019102250A . 2020-07-28

  机译： 从囊肿阶段的卤虫获得自由漂浮的卤虫无节幼体并用于水产养殖的方法

• 4. Method for obtaining free-floating Artemia nauplii from Artemia at the stage of cysts and set for aquaculture [P] . 俄罗斯专利： RU2019102250A3 . 2020-07-28

  机译： 从囊肿阶段的卤虫获得自由漂浮的卤虫无节幼体并用于水产养殖的方法

• 5. Method for producing free swimming Artemia nauplii and packaged cysts for use in that method [P] . 韩国专利： KR100794876B1 . 2008-01-14

  机译： 生产自由泳卤虫无节幼体和用于该方法的包装囊肿的方法

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Enrichment of iArtemia/i(Leach) nauplii with Canola Oil: Effect on iHeros severus/i(Heckel) Larvae Performance and Environmental Stress [J] . Javad Motamedi Tehrani ,Eisa Ebrahimi Dorche ,Sayed Amir Hosein Goli . 微生物学（英文） . 2014,第16期
• 3. 孵化盐度对卤虫无节幼体冷水性淡水水体的中短期存活效应的影响 [C] . 何相燕 ,王智超 ,刘安 . 新疆动物学会2014年年会暨学术研讨会 . 2014
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