首页> 外国专利> WELL TOLERABLE AND HIGHLY SPECIFIC ADAPTED RECOMBINASE FOR RECOMBINATION OF ASYMMETRIC TARGET REGIONS IN VARIETY OF STRAINS OF RETROVIRUSES

WELL TOLERABLE AND HIGHLY SPECIFIC ADAPTED RECOMBINASE FOR RECOMBINATION OF ASYMMETRIC TARGET REGIONS IN VARIETY OF STRAINS OF RETROVIRUSES

机译:适应性强,适应性强的重组重组酶用于各种反转录病毒株的非对称靶区域重组

摘要

The present invention relates to a method for preparing an expression vector encoding a well-tolerated and highly specific tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains which may be inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with these, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection, in particular, HIV infection. In particular, the invention relates to well-tolerated and highly specific tailored recombinases capable of combining asymmetric target sequences in a more than 90% of HIV-1 strains, thereby excising the HIV-1 sequences, and expression vectors encoding them.
机译:本发明涉及一种制备编码载体的方法,该表达载体编码耐受良好且高度特异性的重组酶,该重组酶能够在多个逆转录病毒株的前病毒DNA的长末端重复序列(LTR)内重组不对称靶序列。可将其插入宿主细胞的基因组以及所获得的表达载体中,将其转染的细胞表达重组酶以及包含该表达载体,细胞和/或重组酶的药物组合物。药物组合物可用于例如治疗和/或预防逆转录病毒感染,特别是HIV感染。特别地,本发明涉及能够在超过90%的HIV-1菌株中结合不对称靶序列,从而切除HIV-1序列的耐受良好且高度特异性的重组酶,以及编码它们的表达载体。

著录项

  • 公开/公告号RU2016148309A3

    专利类型

  • 公开/公告日2019-04-03

    原文格式PDF

  • 申请/专利权人

    申请/专利号RU2016148309

  • 发明设计人

    申请日0000-00-00

  • 分类号C12N9/22;C12N15/10;

  • 国家 RU

  • 入库时间 2022-08-21 11:47:13

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