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ULTRA-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETECTION METHOD FOR TRADITIONAL CHINESE MEDICINE COMPOSITION
ULTRA-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETECTION METHOD FOR TRADITIONAL CHINESE MEDICINE COMPOSITION
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机译：中药成分的超高效液相色谱检测方法
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摘要
An ultra-high-performance liquid chromatography detection method for a traditional Chinese medicine (TCM) composition. The detection method comprises the following steps: step A, preparation of a test sample solution: collecting the content of a TCM composition capsule and adding an organic solvent for extraction so as to obtain the test sample solution; step B, preparation of a control solution: collecting chlorogenic acid, caffeic acid, rutin, isochlorogenic acid B, isochlorogenic acid C, forsythiaside A and forsythin and adding an organic solvent to dissolve so as to obtain the control solution; and step C, detection: using ultra-high-performance liquid chromatography with methanol (A) and water containing phosphate (B) as mobile phases for gradient elution. The ultra-high-performance liquid chromatography can quantitatively determine seven chemical components in a composition, such that the quality of the composition is evaluated by means of said fast multi-component chemical analysis, thereby achieving better characterization of quality differences between batches of TCM composition capsules.
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