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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >A rapid method for simultaneous determination of zearalenone, α-zearalenol, β-zearalenol, zearalanone, α-zearalanol and β-zearalanol in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry
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A rapid method for simultaneous determination of zearalenone, α-zearalenol, β-zearalenol, zearalanone, α-zearalanol and β-zearalanol in traditional Chinese medicines by ultra-high-performance liquid chromatography-tandem mass spectrometry

机译：超高效液相色谱-串联质谱法同时测定中药中的玉米赤霉烯酮，α-玉米赤霉烯醇，β-玉米赤霉烯醇，玉米赤霉烯酮，α-玉米醇和β-玉米醇

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摘要

A rapid and reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of zearalenone (ZEN), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalanone (ZAN), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL) in traditional Chinese medicines (TCMs) was developed. The development of the method and investigations of the matrix influence were described in particular. After evaluation of the matrix effects of different TCMs, i.e., rhizomes and roots, seeds, flowers, grasses and leaves, by the post-extraction spiked method, a reliable sample clean-up method based on home-made clean-up cartridges, a suitable internal standard and the matrix calibration were combined using to minimize the matrix effects to ensure the accuracy of the method. The established method was further validated by determining the linearity (R~2≥0.9990), sensitivity (limit of quantitation 0.11-0.99ngmL~(-1)), average recovery (86.6-113.5%) and precision (relative standard deviation ≤13.5%). It was shown to be a suitable method for simultaneous determination of ZEN, α-ZOL, β-ZOL, ZAN, α-ZAL and β-ZAL in different TCMs. Finally, the established method was successfully applied to the determination of the six mycotoxins in various TCMs and the results were presented to provide relevant insights to researchers in TCM analysis.
机译：快速可靠的超高效液相色谱-串联质谱（UHPLC-MS / MS）方法同时测定玉米赤霉烯酮（ZEN），α-玉米赤霉烯醇（α-ZOL），β-玉米赤霉烯醇（β-ZOL），开发了中药（TCMs）中的Zearalanone（ZAN），α-zearalanol（α-ZAL）和β-zearalanol（β-ZAL）。特别描述了该方法的发展以及对矩阵影响的研究。在通过萃取后加标方法评估了不同中药（如根茎和根，种子，花，草和叶）的基质效应后，这是一种基于自制净化柱的可靠样品净化方法，使用合适的内标和基质校准相结合，以最大程度地减少基质效应，以确保方法的准确性。确定线性（R〜2≥0.9990），灵敏度（定量限0.11-0.99ngmL〜（-1）），平均回收率（86.6-113.5％）和精密度（相对标准偏差≤13.5）进一步验证了所建立的方法％）。它被证明是同时测定不同中药中ZEN，α-ZOL，β-ZOL，ZAN，α-ZAL和β-ZAL的合适方法。最后，所建立的方法已成功地用于测定各种中药中的6种真菌毒素，并为研究人员提供了有关中药分析的相关见解。

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