According to the principle of zearalenone (ZEN) dissolving in ethanol easily,the corn germ meal was washed by ethanol to remove ZEN,improve protein content and sensory quality.With corn germ meal as raw material,the effects of volume fraction of ethanol,ethanol washing temperature,ratio of material to liquid,ethanol washing time and ethanol washing times on the removal effect of ZEN were studied.Based on single factor experiment,the optimal removal conditions of ZEN from corn germ meal by ethanol washing method were obtained by orthogonal experiment as follows:volume fraction of ethanol 80％,ethanol washing temperature 40 ℃,ratio of material to liquid 1∶ 15,ethanol washing time 50 min and ethanol washing once.Under these conditions,the content of ZEN in the corn germ meal after ethanol washing decreased from 906.60 μg/kg to 179.21 μg/kg,which was less than 500 μg/kg required by national standard to forage,and the removal rate of ZEN reached above 80％.In addition,the protein content increased from 17.3％ to 21.8％,and the sensory qualities such as color and flavor were improved.%根据玉米赤霉烯酮(ZEN)易溶解于乙醇的原理,利用乙醇对玉米胚芽粕进行浸洗,脱除其中ZEN并提高蛋白质含量和改善感官品质.以玉米胚芽粕为原料,研究乙醇体积分数、醇洗温度、料液比、醇洗时间、醇洗次数对ZEN脱除效果的影响.在单因素试验的基础上,采用正交试验研究确定醇洗法脱除玉米胚芽粕中ZEN的最佳工艺条件为:乙醇体积分数80％,醇洗温度40℃,料液比1∶15,醇洗时间50 min,醇洗次数1次.在最佳工艺条件下,醇洗后玉米胚芽粕中ZEN含量从906.60μg/kg降低至179.21 μg/kg,达到国标对饲料粕500 μg/kg限量要求,ZEN脱除率达到80％以上,醇洗玉米胚芽粕中蛋白质含量从17.3％提高至21.8％,且色泽、风味等感官品质得到改善.
展开▼
