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DETECTION METHOD FOR RAPIDLY IDENTIFYING PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS VACCINE STRAIN AND OTHER VIRULENT STRAINS
DETECTION METHOD FOR RAPIDLY IDENTIFYING PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS VACCINE STRAIN AND OTHER VIRULENT STRAINS
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机译:快速鉴定猪繁殖与呼吸综合征病毒疫苗株和其他毒株的检测方法
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摘要
Disclosed is a PCR-HRM detection method for rapidly identifying a PRRSV vaccine strain, i.e. GDr180 strain, and other virulent strains. The method of the present invention is based on PCR in combination with HRM, comprising a first round of PCR conventional amplification and a second round of PCR-HRM, and the addition of a fluorescence-saturated dye before the reaction.
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