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Purification of polypeptides using two-phase tangential flow ultrafiltration
Purification of polypeptides using two-phase tangential flow ultrafiltration
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机译：使用两相切向流超滤纯化多肽
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摘要
A method for separating a protein of interest from a cell culture solution containing said protein using two-phase tangential flow ultrafiltration (TFF), wherein said two-phase TFF comprises a first TFF unit run and a second unit run TFF, wherein said first TFF unit run filters a first product stream containing said protein using a first ultrafiltration membrane having a cutoff value such that said protein is recovered in the retentate from the first TFF unit run ; and said second TFF unit run filters a second product stream containing said protein using a second ultrafiltration membrane having a cut-off value such that said protein is recovered in the permeate from the second TFF unit run; and wherein said first or second stream of product is a cell culture supernatant.
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