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首页> 外文期刊>International Journal of Molecular Sciences >Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment
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Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment

机译：切向流超滤允许纯化和浓缩月桂酸/白蛋白涂层的颗粒，以改善磁性处理

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Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEONLA-BSA, which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEONLA-BSA particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEONLA-BSA changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic hyperthermia is greatly enhanced from 44.4 °C up to 64.9 °C by the concentration of the particles up to 16.9 mg/mL total iron. Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.
机译：超顺磁性氧化铁纳米颗粒（SPIONs）通常用于药物靶向，热疗和其他生物医学目的。最近，我们报道了使用过量白蛋白合成月桂酸/白蛋白涂层的氧化铁纳米颗粒SEON LA-BSA 的合成。为了优化磁处理应用，需要将SPION悬浮液中多余的表面活性剂纯化并浓缩。纯化和浓缩此类铁磁流体的常规方法通常涉及高剪切应力和大分子（如白蛋白）的低纯化率。研究了低切应力切向超滤去除白蛋白及其对SEON LA-BSA 颗粒的影响。通过过滤或浓缩至四倍（v / v），纳米颗粒的流体力学尺寸，表面性质以及因此的胶体稳定性保持不变。因此，悬架的饱和磁化强度可以从446.5 A / m增加到1667.9 A / m。体外分析表明，SEON LA-BSA 的细胞摄取仅发生少量变化。比吸收率（SAR）不受浓度影响很大。相比之下，磁热疗的最高温度T max 通过总铁含量高达16.9 mg / mL的颗粒浓度而大大提高，从44.4°C升高到64.9°C。总之，切向超滤对于纯化和浓缩复杂的杂化包被的SPION悬浮液是可行的，而不会负面影响特定的颗粒特性。这增强了他们进行磁疗的潜力。

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