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Expression of nitrogenase polypeptides in plant cells

机译:固氮酶多肽在植物细胞中的表达

摘要

The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom.
机译:本发明涉及在植物细胞的线粒体中产生固氮酶多肽的方法和手段。本公开提供了表达一种或多种MTP-Nif融合物和/或翻译的NifD-NifK和NifE-NifN融合物的植物细胞。本公开还提供了编码这些融合体的核酸构建体,以及用于表达融合体并将其靶向于植物细胞的线粒体的表达构建体。本公开还提供了包含本发明的植物细胞和从其获得的产物的转基因植物。

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