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GLYCAN ANALYSIS METHOD, GLYCAN ANALYSIS SYSTEM, PROGRAM FOR GLYCAN ANALYSIS, AND KIT FOR GLYCAN ANALYSIS
GLYCAN ANALYSIS METHOD, GLYCAN ANALYSIS SYSTEM, PROGRAM FOR GLYCAN ANALYSIS, AND KIT FOR GLYCAN ANALYSIS
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机译：糖分析方法，糖分析系统，糖分析程序和糖分析试剂盒
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摘要
A sample, which is a mixture of glycans, is fluorescently labeled (S2). The sample is subsequently separated by microchip electrophoresis under a buffer solution with no lectin added as well as under multiple kinds of buffer solutions with different lectins respectively added, and the separated components are fluorescently detected (S3). A high-concentration gel which can produce a molecular-sieving effect is used as the buffer solution. Multiple electropherograms are created from the detection results (S4). A glycan having a lectin specifically attached is delayed during its migration in the buffer solution, so that a peak corresponding to this glycan will effectively disappear. Accordingly, based on the kinds of lectins and the presence/absence of a peak on each of the electropherograms, the structure of each glycan in the sample is estimated and the glycan is identified (S5).
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