首页> 外国专利> METHOD OF DIFFERENTIATING NEURAL STEM CELLS OR NEURAL PRECURSOR CELLS INTO DOPAMINE NEURONS

METHOD OF DIFFERENTIATING NEURAL STEM CELLS OR NEURAL PRECURSOR CELLS INTO DOPAMINE NEURONS

机译:将神经干细胞或神经前体细胞分化为多巴胺神经元的方法

摘要

The present invention relates to a method of differentiating neural stem cells or neural precursor cells into dopamine neurons and more particularly, a method of differentiating into dopamine neurons, in which chromosomal stability is maintained by transfecting neural stem cells or neural precursor cells with mRNA of a dopamine neuron-inducing transcription factor under time-based control. The method of differentiating into dopamine neurons, according to the present invention, may enable preparation of mature and functional dopamine neurons having chromosomal stability by synthesizing a dopamine neuron-inducing transcription factor into a mRNA form, which has no risk of genetic modification, and transfecting the synthetic mRNA, unlike existing methods using retroviral vectors, and thus may be usefully used in the clinical field for the treatment of Parkinson's disease.
机译:本发明涉及将神经干细胞或神经前体细胞分化为多巴胺神经元的方法,更具体地,涉及分化为多巴胺神经元的方法,其中通过用α1的mRNA转染神经干细胞或神经前体细胞来维持染色体的稳定性。基于时间的控制下的多巴胺神经元诱导转录因子。根据本发明的分化为多巴胺神经元的方法可以通过将多巴胺神经元诱导转录因子合成为mRNA形式的mRNA形式而制备具有染色体稳定性的成熟的和功能性的多巴胺神经元,其没有遗传修饰的风险并转染。不同于使用逆转录病毒载体的现有方法,合成的mRNA因此可在临床领域中有效地用于治疗帕金森氏病。

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