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METHOD OF DIFFERENTIATING NEURAL STEM CELLS OR NEURAL PRECURSOR CELLS INTO DOPAMINE NEURONS

机译:将神经干细胞或神经前体细胞分化为多巴胺神经元的方法

摘要

To provide a method of differentiating neural stem cells or neural precursor cells into dopamine neurons.SOLUTION: More particularly, a method of differentiating into dopamine neurons is provided, in which chromosomal stability is maintained by transfecting neural stem cells or neural precursor cells with mRNA of a dopamine neuron-inducing transcription factor under time-based control. The method of differentiating into dopamine neurons, according to the present invention, may enable preparation of mature and functional dopamine neurons having chromosomal stability by synthesizing a dopamine neuron-inducing transcription factor into a mRNA form, which has no risk of genetic modification, and transfecting the synthetic mRNA, unlike existing methods using retroviral vectors, and thus may be usefully used in the clinical field for the treatment of Parkinson's disease.SELECTED DRAWING: Figure 6b
机译:提供一种将神经干细胞或神经前体细胞分化为多巴胺神经元的方法。解决方案:更具体地讲,提供了一种分化为多巴胺神经元的方法,其中通过将神经干细胞或神经前体细胞以在基于时间的控制下诱导多巴胺神经元的转录因子。根据本发明的分化为多巴胺神经元的方法可以通过将多巴胺神经元诱导转录因子合成为mRNA形式的mRNA形式而制备具有染色体稳定性的成熟的和功能性的多巴胺神经元,其没有遗传修饰的风险并转染。合成的mRNA,与使用逆转录病毒载体的现有方法不同,因此可以在临床领域中有效地用于治疗帕金森氏病。选图:图6b

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