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Fluorescent probe, fluorescent detection method and method of using fluorescent probe
Fluorescent probe, fluorescent detection method and method of using fluorescent probe
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机译：荧光探针，荧光检测方法及使用该荧光探针的方法
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摘要
The present invention includes a carrier molecule, a fluorescent dye a and a fluorescent dye b bonded to the carrier molecule, the fluorescent dye a and the fluorescent dye b have different excitation wavelengths, and the fluorescent light FRET between the fluorescent dye a and the fluorescent dye b. The present invention relates to a fluorescent probe that does not cause the problem The present invention also relates to a fluorescence detection method comprising a step of labeling a target cell with the fluorescent probe and a step of irradiating the target cell labeled with the fluorescent probe with excitation light and observing fluorescence from the fluorescent probe. . The present invention also includes a step of fluorescently labeling cells with the fluorescent probe, a step of selecting fluorescently labeled cells with a flow cytometer or a fluorescent microscope, a step of transplanting the selected fluorescently labeled cells into a living body, The present invention relates to a method for using a fluorescent probe, which comprises the step of observing fluorescently labeled cells in the body with an in vivo fluorescence imaging apparatus.
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