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Method for evaluating and selecting induced pluripotent stem cells, and method for producing induced pluripotent stem cells
Method for evaluating and selecting induced pluripotent stem cells, and method for producing induced pluripotent stem cells
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机译:评估和选择诱导多能干细胞的方法以及产生诱导多能干细胞的方法
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摘要
The present invention provides a step of providing a cultured induced pluripotent stem cell, a step of measuring the natural appearance frequency of an organelle having an abnormal nucleic acid structure in the provided induced pluripotent stem cell, and the natural appearance frequency as a reference Method for evaluating induced pluripotent stem cells including a step of discriminating induced pluripotent stem cells that are less than or equal to induced pluripotent stem cells whose natural occurrence frequency exceeds the reference value, and further, the natural occurrence frequency is lower than the reference value There is provided a method for selecting induced pluripotent stem cells comprising a step of selecting induced pluripotent stem cells. Furthermore, the present invention provides a step of providing an established and cultured induced pluripotent stem cell, a step of measuring the natural appearance frequency of an organelle having an abnormal nucleic acid structure in the provided induced pluripotent stem cell, A step of discriminating between induced pluripotent stem cells whose appearance frequency is below the reference value and induced pluripotent stem cells whose natural appearance frequency exceeds the reference value, and induced pluripotent stem cells whose natural appearance frequency is below the reference value Provided is a method for producing induced pluripotent stem cells including a step of selecting.
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