To present a method for visualizing the interaction of hair protein and water by water diffusion to the inside of hair by continuously evaluating the spectral change of hair protein that is caused by heavy water diffusing from cuticle to the inside of hair.SOLUTION: Provided is a method for measuring the interaction of hair protein and water by water diffusion, in which the signal strength of deuterium-substituted amide II near 1540 cmis measured out of the infrared absorption spectrums measured using a measurement method that includes a step for bringing the cross-sectional cut piece of hair into contact with heavy water after sandwiching it with cells transparent to infrared rays and a step for measuring the target substance by infrared absorption spectroscopy.SELECTED DRAWING: Figure 1
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