首页> 外国专利> LIVER-TYPE FATTY ACID-BINDING PROTEIN PREPARATION, METHOD FOR EVALUATING PREPARATION, METHOD FOR REGULATING VARIATION RANGE OF MEASURED VALUE CAUSED BY LIVER-TYPE FATTY ACID-BINDING PROTEIN IN MEASUREMENT USING PREPARATION, LIVER-TYPE FATTY ACID-BINDING PROTEIN, DNA ENCODING PROTEIN, CELL TRANSFORMED BY DNA, METHOD FOR PRODUCING PROTEIN, METHOD FOR DRAWING CALIBRATION CURVE FOR LIVER-TYPE FATTY ACID-BINDING PROTEIN, AND METHOD FOR QUANTIFYING PROTEIN

LIVER-TYPE FATTY ACID-BINDING PROTEIN PREPARATION, METHOD FOR EVALUATING PREPARATION, METHOD FOR REGULATING VARIATION RANGE OF MEASURED VALUE CAUSED BY LIVER-TYPE FATTY ACID-BINDING PROTEIN IN MEASUREMENT USING PREPARATION, LIVER-TYPE FATTY ACID-BINDING PROTEIN, DNA ENCODING PROTEIN, CELL TRANSFORMED BY DNA, METHOD FOR PRODUCING PROTEIN, METHOD FOR DRAWING CALIBRATION CURVE FOR LIVER-TYPE FATTY ACID-BINDING PROTEIN, AND METHOD FOR QUANTIFYING PROTEIN

机译：肝型脂肪酸结合蛋白的制备，评估方法，调节制备中肝型脂肪酸结合蛋白测定值的变化范围的方法，肝型脂肪酸结合蛋白，DNA编码，DNA转化的细胞，蛋白质的制备方法，肝型脂肪酸结合蛋白的标定曲线绘制方法以及蛋白质的定量方法

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摘要

The purpose of the present invention is to provide: a liver-type fatty acid-binding protein standard by which, in a measurement using a specifically binding substance, the range of variation of a measured value caused by a liver-type fatty acid-binding protein can be narrowed; a method of evaluating the standard; a method of drawing a calibration curve of a liver-type fatty acid-binding protein; and a method of quantifying the protein. A liver-type fatty acid-binding protein standard in which a coefficient of change in oxidation, said coefficient being represented by the ratio of a measured value obtained by using a liver-type fatty acid-binding protein standard having been subjected to an oxidation treatment with 10 mM of an oxidant for 1 hour at 25°C to a measured value obtained by using the liver-type fatty acid-binding protein standard not subjected to the oxidation treatment, is set to 1.4 or less; a liver-type fatty acid-binding protein to be used in the standard; a DNA encoding the protein; a cell transformed by the DNA; a method of producing the protein; a method of evaluating the standard; a method of regulating the variation range of a measured value in a measurement using the standard; a method of drawing a calibration curve for the protein; and a method of quantifying the protein.

机译：本发明的目的是提供：肝型脂肪酸结合蛋白标准品，通过该标准品，在使用特异性结合物质的测定中，由肝型脂肪酸结合引起的测定值的变化范围蛋白质可以变窄;评估标准的方法;绘制肝脏型脂肪酸结合蛋白的校准曲线的方法;和定量蛋白质的方法。一种肝型脂肪酸结合蛋白标准品，其中氧化变化系数表示为通过使用经过氧化处理的肝型脂肪酸结合蛋白标准品获得的测量值的比值在25℃下用10mM的氧化剂在1小时内，将使用未经氧化处理的肝型脂肪酸结合蛋白标准品获得的测量值设为1.4以下。标准中使用的肝型脂肪酸结合蛋白;编码蛋白质的DNA;由DNA转化的细胞;生产蛋白质的方法;评估标准的方法;使用该标准来调节测量中的测量值的变化范围的方法;绘制蛋白质校准曲线的方法;和定量蛋白质的方法。

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公开/公告号EP3421600A4

专利类型
公开/公告日2019-03-20

原文格式PDF
申请/专利权人 CMIC HOLDINGS CO. LTD.;
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申请/专利号EP20170813408
发明设计人 SUGAYA TAKESHI;OKAZAKI MASATERU;OIKAWA TSUYOSHI;
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申请日2017-06-15
分类号C12N15/09;C07K14/47;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12P21/02;G01N33/68;
国家 EP
入库时间 2022-08-21 12:29:07

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